GC-MS Analysis on Volatiles of <i>Iris lactea</i> var. <i>chinensis</i> at Different Flowering Stages

ZHU Ying; SUN Junming; LOU Qian; CHEN Hongwu

doi:10.19303/j.issn.1008-0384.2023.07.004

Volume 38 Issue 7

Jul. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(7): 792-799

ZHU Y, SUN J M, LOU Q, et al. GC-MS Analysis on Volatiles of Iris lactea var. chinensis at Different Flowering Stages [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：792−799 doi: 10.19303/j.issn.1008-0384.2023.07.004

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ZHU Y, SUN J M, LOU Q, et al. GC-MS Analysis on Volatiles of Iris lactea var. chinensis at Different Flowering Stages [J]. Fujian Journal of Agricultural Sciences，2023，38（7）：792−799 doi: 10.19303/j.issn.1008-0384.2023.07.004

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GC-MS Analysis on Volatiles of Iris lactea var. chinensis at Different Flowering Stages

doi: 10.19303/j.issn.1008-0384.2023.07.004

1.
Beijing Botanical Garden, Beijing　100093, China
2.
College of Horticulture, Northwest A & F University, Yangling, Shannxi 712100, China

Received Date: 2022-12-13
Rev Recd Date: 2023-03-15

Available Online: 2023-07-06

Publish Date: 2023-07-28

Abstract

Abstract

Objective Floral fragrance and release of Iris at different stages were analyzed using GC-MS. Methods Volatiles in the flowers of Iris lactea var. chinensis (Fisch.) Koidz at budding, blooming, and fading stages were determined by HS-SPME-GC-MS. Result The analysis identified 69 distinct aromatics. The continuously released volatiles in the entire flowering period were mainly ketones, terpenes, and aldehydes. There were also alcohols, esters, as well as in smaller quantities of alkanes, benzenoids, and nitrogenous compounds. At different flowering stages, hexanal, cyclohexanone, 5-hepten-2-one, 6-methyl- and β−irone were detected in the buds, hexanal, (−)-β−pinene, and 5-hepten-2-one, 6-methyl- in the blooms, and (-)-β−pinene, 5-hepten-2-one, 6-methyl-, hexanal, phenylethyl alcohol, and cyclohexanone in the fading flowers. The OPLS-DA showed differentiations on heptane, 3-methyl-, 5-hepten-2-one, 6-methyl-, (−)-β−pinene, hexanoic acid, ethyl ester, α−pinene, 3-carene, linalool, phenethyl alcohol, decanoic acid, ethyl ester, and β−irone at these stages. The calculated odor activity values (OAVs) on pyrazine, 2-methoxy-3-(1-methylpropyl)-, hexanoic acid, ethyl ester, linalool, nonanal, hexanal, 2-nonenal, and (E)- indicated that these were the characteristic aromatic substances of I. lactea var. chinensis and that pyrazine and 2-methoxy-3-(1-methylpropyl)- significantly greater in quantity (P＜0.01). Conclusion For the first time, the chemical composition of the fragrance of I. lactea var. chinensis flowers was analyzed, and compounds identified in detail. The released volatiles differed significantly during the floral development. It was at the fading stage when the fragrance peaked with the major aromatic contributors including hexanal, 5-hepten-2-one, 6-methyl-, cyclohexanone, and phenethyl alcohol.
- Iris lactea var. chinensis (Fisch.) Koidz,
- flowering period,
- volatiles,
- GC-MS

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References(32)

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