Abstract: The ectoenzyme activity and the virulence against Planococcus citri of Metarhizium anisopliae F061 were determined in the laboratory. Their correlation was established using a linear regression analysis. The activities of two ectoenzymes from the bacteria were high.At concentrations of 1.00×10⁸ and 1.00×10⁷ conidia·mL^-1, the protease activities of the spores were 7.63 U·mL^-1 and 7.61 U·mL^-1, respectively; while the chitinase activities, 5.45 U·mL^-1 and 5.67 U·mL^-1, respectively; and, the LT₅₀ of the strain against P.citri, 4.98 d and 5.39 d, respectively. The LT₅₀ inversely correlated significantly with the protease activity, but moderately with the chitinase activity. It appeared that the protease were stronger than the chitinase in M.anisopliae F061, and thus, played a more critical role in infecting P. citri.