Abstract: The seed-derived calli and microspore-derived calli of rice were cultured on the medium supplemented with high concentration oi lysine, thrrouino or tryptophane for screening in vitro. Some resistants to high concentration of amino acid calli were isolated and then regenerated into green plants. By analysing the amino acid contents of brown rice in T1 generation, the total amount of 17 kinds of amino acid in 9 mutants was increased by 10.6-46.9%, the amount of 1ys, Thr, Met, Phe, Leu, Ileu and Val plus Cys was increased by 10.4-58.2%, among them, the contents of lysine was increased by 7.8-42.3%. With counting the C. V. of main agronomic traits, the fertile mutants in T1-T2 were stable. The possible potential of screening resistant mutants in vitro used for rice quality breeding was discussed.