Abstract: By varying the breathable plastic bags,formulation of mother spawns,moisture levels of the wheat,external physical and chemical characteristics of the wheat,and antibiotics added to the wheat,the mass producing of mushroom spawns was optimized.The results showed that(a)T type breathable bags was suitable for the application;(b)the mineral mother spawn was the best;(c)moisture level should be in the range of 42% to 45% to yield the highest production rate;(d)cooking wheat with 0.3% quicklime,2% gypsum and 4% calcium carbonate resulted in a alkaline coat on the wheat,which is conducive to mycelium growth and improves its competitiveness to other microorganisms;and,(e)cooking wheat with 0.02% benzimidazole 44 and 0.025% streptomycin sulfate prevented spawns from mold and bacterial infections during incubation,while being harmless to the mycelium development.