Abstract: Shoot tips from Liriope muscari (Desne.) Bailey were used as explants to grow on media with varied sterilization time,under induced differentiation,as well as different strengthening and rooting treatments, and transplantation conditions. The shoot tips were cut from the plants and cultured on MS medium containing 2.0 mgL-1 BA and 0.2 mgL-1 NAA. The highest differentiating rate of 90% was achieved. The medium with 4.0 mgL-1BA and 0.2 mgL-1 NAA was suitable for multiple-shoot proliferation. A certain concentrations of BA promoted the shoot differentiation. The optimized medium for seeding and rooting was when MS was mixed with 0.5 mgL-1 IBA, which improved the root coefficient to 6.87. Peat soil was found to be the best medium material for the transplantation.