提高耐盐红螺菌拮抗能力的发酵条件初探

A preliminary study on the improvement of antagonistic efficiency of salt-resistant Rhodospirillaceae

摘要: 以光合细菌培养基为基础,初步探讨提高耐盐红螺菌拮抗能力的培养基组分等发酵条件,结果表明培养基的最佳碳源为葡萄糖,最佳氮源为磷酸氢二铵,并在培养基中应缺省硫酸锰和氯化钙。以优化后的培养基培养耐盐红螺菌,最低抑菌浓度达32 AU·mL-1,较原光合细菌基础培养基提高了大约1倍。添加聚乙二醇400、D-山梨醇表面活性剂不能提高该菌的拮抗能力。

Abstract: Culture conditions to improve the antagonistic efficiency of the bacteria were investigated using the photosynthetic bacteria medium.It was found that the optimum carbon source was glucose,the optimum nitrogen source(NH4)2HPO4,and MnSO4 H2O and CaCl2 to be eliminated in the medium.The minimum inhibitory concentration on the Rhodospirillaceae under the optimized conditions was 32 AU ml-1,doubled from the original medium.However,there was no effect on the antagonism by the addition of surfactants PEG and D-sorbitol.