微核监测技术在蚕豆种质资源耐盐性评价中的应用研究

Application of micronucleus test technology to salt-tolerance evaluation in broadbean gepmplasm resources

摘要: 研究了100份蚕豆种质的根尖细胞微核率及盐敏指数差异情况,以盐胁迫发芽试验的相对盐害率为参照指标,分析微核率、盐敏指数和相对盐害率间的相关关系。结果表明:微核监测技术适用于蚕豆种质的耐盐性鉴定,供试的100份蚕豆种质在3.0%NaCl盐溶液胁迫下的微核率变异幅度为8.3～15.3,盐敏指数变异幅度为0.33～2.56;以盐敏指数为耐盐指标,对上述100份蚕豆种质资源进行耐盐力分级,其中Ⅰ级占3%、Ⅱ级占9%、Ⅲ级占18%、Ⅳ级占32%、Ⅴ级占38%。

Abstract: The difference of root-tip cell micronucleus frequency and salt-sensitive index among 100 Vicia faba varieties were studied.Correlativity among micronucleus frequency,salt-sensitive index and relatively salt damage rate were analyzed by the relatively damage rate of seedlings under salt-stress.Micronucleus test technology was adapted to appraise salt-tolerance of Vicia faba,the Vicia faba micronucleus frequency were 8.3 to 15.3,the salt-sensitive index were 0.33 to 2.56 under 3.0% NaCl.Salt-tolerance of 100 Vacia faba varieties was divided into 5 grades by salt-sensitive index,among whith,3 in Ⅰ,9 in Ⅱ,18 in Ⅲ,32 in Ⅳ and 38 in Ⅴ.