摘要:
以黑龙江省正茬土壤为研究对象,采用室内回接培养的方法,研究了大豆根系分泌物中2种有机酸(苹果酸、酒石酸)和3种酚酸(油酸甲酯、2,4-二叔丁基苯酚和香草酸)对土壤微生物量和微生物活性的影响,并通过测定土壤微生物C/N比,初步确定有显著作用的分泌物组分。结果表明,苹果酸、酒石酸和油酸甲酯的加入对微生物来说是有益的,化感作用并没有表现出来;而2,4-二叔丁基苯酚和香草酸的加入,对土壤微生物的生长表现为低浓度促进高浓度抑制,对土壤微生物有化感作用。
Abstract:
This study aimed to determine the relationship between allelopathy and soil microorganisms in order to find out the possible barriers that inhibit continuous cropping of soybeans.By altering two organic acids(i.e.,malic and tartaric acid)and three phenolic acids(i.e.,oleic acid methyl ester,2,4-dinitrophenol and vanillic acid)among the known soybean root exudates,the effect on the biomass and activity of the microbes in soil was determined.Using the soil sample collected from the continuous cropping field in Heilongjiang province,the in-lab frankia culture method was employed and the carbon/nitrogen ratio of the soil microbial biomass measured to preliminary identify the major components in the exudate.The results indicated that the addition of malic acid,tartaric acid and oleic acid methyl ester benefitted the microbial growth without allelopathy effect.On the other hand,the addition of2,4-dinitrophenol and vanillic acid at low concentrations increased the microbial biomass,while reduced it at high concentrations.The results suggested that 2,4-dinitrophenol and vanillic acid might play an important role in the soybean allelopathy.
百度学术
下载:
