2023 Vol. 38, No. 2
Display Method:
2023, 38(2): 127-136.
doi: 10.19303/j.issn.1008-0384.2023.02.001
Abstract:
Objective Shape and quality of the grains of Fuhui 676, a premium high-yield, high-quality, stress resistant, highly adaptable restorer of rice, were studied for further improvements. Method Along with Fuhui 676, which was previously bred by the Rice Research Institute at Fujian Academy of Agricultural Sciences, Minghui 63, Shuhui 527, Minghui 86, and Fuhui 673 were planted at the experimental stations in Fuzhou, Quanzhou, and Sanming. At maturation, grains were harvested and sun-dried to measure the length, width, thickness, 1000-grain weight, and quality. Genomic DNA of the plants was extracted by CTAB method; Wx fragments amplified by PCR; restriction enzymes analyzed by means of Acc I digestion; polymorphism of the first base of Wx gene intron 1 determined by agarose gel electrophoresis and sequence analysis; total RNA of the grains extracted by Trizol method; and expressions of the grain-shape-and-quality-related genes at different grain-filling stages analyzed by SYBR Green I fluorescent quantitative PCR (qRT-PCR). Result In general, the Fuhui 676 grains measured 10–11 mm long, 2.70 mm wide, 2.00 mm thick, and 27.0–31.0 g per 1000-grains. After dehulling, they yielded more than 81% brown rice with a gel consistency of more than 60.0 mm and an amylose content in the range of 13%–18%, which met the 1st grade standard. The grains had a higher head rice rate than those of the other 4 restorer lines; and the chalkiness rate and degree higher than those of the parents, Minghui 63 and Shuhui 527, but lower than those of Minghui 86 and Fuhui 673. The first base of Wx gene intron 1 in the restorers was T, i.e., of the Wxb genotype. The expression of GL7 related to grain shape in Fuhui 676 decreased significantly on the 11 d of grain-filling, while those of GS3, SGL, and GIF1 increased initially and then decreased with a peak appeared on the 7 d; that of GW8 decreased at first and then increased to the highest level on the 11 d; that of the quality-related Chalk5 reached the maximum on the 7 d but nearly dissipated on the 11 d; and those of ALK, OsSSI, and OsBEIIb rose to a peak on the 7 d and followed by a decline. The expression patterns of those genes in Fuhui 676 were completely different from those in the other 4 restorers. Conclusion Fuhui 676 produced long grains of high head milled rice rate, brown rice rate, gel consistency, and amylose content of 1st grade rice. The Wx genotype of Fuhui 676 was Wxb. The expression patterns of GL7, GS3 and ALK, OsSSI, OsBEIIb related to grain shape and quality in Fuhui 676 were distinctively different from those of the other tested restorer lines.
2023, 38(2): 137-143.
doi: 10.19303/j.issn.1008-0384.2023.02.002
Abstract:
Objective Genome sizes of the economically, medicinally, and ornamentally valuable Ardisia crenata were determined using flow cytometry. Methods Genome sizes of 22 selectively bred cultivars and two wild A. crenata germplasms, along with Lycopersicon esculentum as internal reference, were determined by flow cytometry. Results The sizes ranged from 1.77 Gb to 2.41 Gb averaging 1.87 Gb with the smallest C-value of 1.77 Gb found on Agate Red (Z-17), Kasumi Pearl (Z-20), and Zhu Ta (Z-22), while the largest of 2.41 Gb on Chidan. Variations existed among the germplasms. Conclusion For the first time, the genome sizes of various A. crenata were determined to be available for library construction and genomics studies on the valuable natural resource.
2023, 38(2): 144-150.
doi: 10.19303/j.issn.1008-0384.2023.02.003
Abstract:
Objective Genetic information and functions of trihelix factors in GT-1 critical to the growth, development, and resistance to abiotic stresses and plant growth regulator of tomato plants were investigated. Method Bioinformatic techniques were applied to decipher the bio-evolutionary of GT-1 in tomato. RT-PCR was used to determine the expressions and responses to abiotic stresses and plant growth regulator of the gene. Result (1) In tomato, GT-1 had 3 subfamily members, i.e., SlGT-21, SlGT-24, and SlGT-35. The evolutionary analysis indicated that those members differentiated functionally. (2) The 3 subfamily genes expressed in all tested tomato tissues with the highest level in the fruits, especially during the growing stage. It was speculated that SlGT-21 and SlGT-35 might share some common functionalities. (3) All 3 genes, particularly SlGT-24, could be suppressed by dehydration, and SlGT-21 and SlGT-35 more severely affected by salt stress. (4) The expressions of these genes were inhibited by GA(Gibberellin), EBR(Epihomobrassinolide), and MeJA (Methyl jasmonate); but those of SlGT-24 induced by ABA (Abscisic acid) and ACC(1-Aminocyclopropanecarboxylic acid), and SlGT-35 by ABA. Conclusion Three GT-1 subfamily genes of tomato plants could be regulated by salt and/or dehydration stresses and were sensitive to hormonal stimulations.
2023, 38(2): 151-157.
doi: 10.19303/j.issn.1008-0384.2023.02.004
Abstract:
Objective VcILR1 of blueberry was cloned to analyze the expressions in various plant organs as well as after being treated by gibberellin to decipher its function and association with ovule abortion. Method Using the cDNA of blueberry as the template, VcILR1 was cloned by RT-PCR. Bioinformatics on the amino acid sequence was performed utilizing ProtParam and other relevant tools. Expressions of the gene in the flowers, leaves, stems, fruits, and roots of the plant as well as those in green and ripe fruits after being treated by gibberellin were determined. Result VcILR1 was successfully cloned and found to contain 976 bp ORF encoding 325 amino acids with two conserved structural domains, peptidase_M20 and M20_dimer, a molecular mass of 35364.20 kDa, a theoretical isoelectric point of 5.67, an instability coefficient of 43.57, and a lipid coefficient of 88.55. It was an unstable protein with a hydrophilic mean value of −0.054. A phylogenetic tree analysis showed the gene to closely relate to Camellia sinensis IAA-Leucine Resistant 1-like (ILL) gene. The qRT-PCR analysis revealed that the gene was expressed tissue-specific with the highest in the leaves, and that the gibberellin treatment up-regulated the expression in the fruits. Conclusion The expressions of VcILR1 in the blueberries significantly increased after being treated by gibberellin. It suggested that the plant hormone could stimulate IAA generation in the fruits and might also affect the ovule development.
2023, 38(2): 158-165.
doi: 10.19303/j.issn.1008-0384.2023.02.005
Abstract:
Objective Changes in contents of main pigments and the expressions of related genes in pericarp of eggplant fruit during growth and development were studied concerning the color formation. Method Contents of anthocyanin, chlorophyll, and carotenoid in the fruits of two high-generation eggplant inbred lines, green Qidong and purple-black 8 guo, were measured at 15, 20, 25, 30, and 35 d after pollination. Expressions of the structural genes PAL, CHS, CHI, F3H, DFR, and ANS and the transcription factors MYB75, JAF13, and WD40 related to anthocyanin synthesis, as well as the key gene, HEMA1, in chlorophyll synthesis and PSY1 in carotenoid synthesis were analyzed. Result The contents of 3 categories of pigments in Qidong increased initially followed by a decline during the test period with peaks on the 20 d on anthocyanin, 25 d on chlorophyll, and 20 d on carotenoid. For 8 guo, the anthocyanin peaked on the 25 d, but chlorophyll and carotenoid continuously increased throughout the fruit development. 8 guo had higher relative contents of anthocyanin, lower chlorophyll from 15 d to 25 d, and higher carotenoid between 30 d and 35 d than Qidong. The expressions of anthocyanin synthesis structural genes and transcription factors MYB75, JAF13 and WD40 in Qidong were generally lower and relatively stable than those in 8 guo at different growth and development stages. The expression of anthocyanin synthesis structural genes in 8 guo reached a maximum on the 20 d and then began to decrease, while the expression of transcription factors MYB75 and WD40 rose at first and then decreased, which was similar to the trend observed on the anthocyanin. The expressions of HEMA1 and PSY1 in Qidong and 8 guo differed in the changing patterns of chlorophyll and carotenoid. Conclusion During growth and development, the two eggplant varieties of different colored skins underwent significantly different changes on the 3 pigments. The expressions of the pigment-related genes differed significantly as well, which could be studied from a genetic perspective to reveal the underline mechanisms of the observed color formation and differentiation.
2023, 38(2): 166-173.
doi: 10.19303/j.issn.1008-0384.2023.02.006
Abstract:
Objective Genetic diversity and relationship of Phaseolus lunatus L. germplasms were determined using the morphological and SRAP markers. Method Twenty-six quantitative and 18 quality traits of 22 lima bean germplasms were analyzed based on the morphological as well as the SRAP molecular markers. Result Twenty-eight pairs of SRAP primers were selected to obtain 158 amplified polymorphic bands with an average polymorphism ratio of 77.75%. The two methods applying different markers clustered the 22 varieties into 3 categories. Genetically, Shanghengshan10-2-6 and Xiahengshan 10-3-3 were relatively close. They might have gone through numerous gene exchanges in the past. Conclusion The 22 lima bean germplasms were rich in genetic diversity. The morphological and SRAP molecular markers-based clustering essentially agreed with the classification by the pod size.
2023, 38(2): 174-182.
doi: 10.19303/j.issn.1008-0384.2023.02.007
Abstract:
Objective Chloroplast genome structure and phylogeny of the endangered Impatiens omeiana Hook.f. endemic species in China were studied. Method Gene assembly, annotation, characteristics, sequence duplication, and phylogenetics of I. omeiana were analyzed based on the chloroplast genome sequence using bioinformatic software. Results The genome was 152527 bp consisting of 130 gene, including 86 protein-coding, 8 rRNA, and 36 tRNA genes. It had a GC content of 37% and a conservative tetrad structure with regions of a large single-copy of 83150 bp, a small single-copy of 17903 bp, and two identical reverse repeats of 25 737 bp. Thirteen of the genes had one intron, and two had two. There were 76 SSR sequences detected in the genome mainly of A/T single nucleotides with a length ranging from 10 bp to 91 bp. A total of 50842 codons were found in the entire genome. Among the amino acids, leucine (Leu) was the most numerous and tryptophan (Tyr) the least. A codon bias analysis showed those of 33 RSCU＞1 had A/U on their ends. Using the Neighbor Joining method (NJ), a phylogenetic tree was constructed based on the chloroplast genomes from different plants to show a close relationship of I. omeiana with I. guizhouensis belonging to the subgenus Clavicarpa. Conclusion The chloroplast genome of I. omeiana had a typical tetrad structure of many SSR repeated sequences with A/T single base. Phylogenetically, I. omeiana was a Clavicarpa subgenus. These results provide important molecular information for the phylogenetic status and species identification of I. omeiana.
2023, 38(2): 183-192.
doi: 10.19303/j.issn.1008-0384.2023.02.008
Abstract:
Objective Optimal conditions for the growth of Leucaena leucocephala seedlings in an indoor facility were studied. Methods Selected morphological and physiological indices of L. leucocephala seedlings were monitored for the optimization in a chamber under varied light exposures (i.e., 12, 16, 20, and 24 h·d−1) with the intensity of 100, 200, 300, or 400 μmol·m−2·s−1 in a nutrient solution of a salinity of 7‰, 15‰, 20‰, or 25‰, a N concentration of 7.5, 15, 30, or 60 mmol·L−1, and a P concentration of 0.5, 1, 2, or 4 mmol·L−1. Results The single factor experiment showed the seedling morphological indices increased at first and then decreased with increasing light exposure, light intensity, N or P concentration. After 35 d of cultivation, the average fresh weight of an individual seedling exposed to 20 h·d−1 of light reached (5.61±0.11) g, which was 60.0% higher than that exposed to 12 h·d−1 or 14.6% higher than that exposed to 24 h·d−1. The intensity of light at 200 μmol·m−2·s−1 resulted in a (6.55±0.10) g·plant−1 of fresh seedling, which was 21.5% or 62.1% higher than at 100 μmol·m−2·s−1 or 400 μmol·m−2·s−1, respectively. The nutrient solution containing 15 mmol·L−1 N yielded a fresh seedling weight of (4.32±0.10) g·plant−1, which was 6.1% and 108.6% higher than those at 7.5 and 60 mmol·L−1, respectively, in concentration. Whereas P at 1 mmol·L−1, the fresh seedling weight was (5.65±0.21) g·plant−1, which was 40.9% and 64.7% higher than P at 0.5 mmol·L−1 and 4 mmol·L−1, respectively. And the seedlings exhibited similar patterns under those conditions on chlorophyll content, chlorophyll fluorescence parameters, and antioxidant enzyme activities. On the other hand, the seedlings growing under an increasing salinity displayed decreasing trends on the morphological indices, chlorophyll content, chlorophyll fluorescence parameters, and antioxidant enzyme activities. At 7‰ salinity, the greatest fresh seedling weight of (8.95±0.05) g·plant−1, dry weight of (2.16±0.16) g·plant−1, plant height of (31.17±1.67) cm, root length of (60.67±0.93) cm, and total chlorophyll content of (1.72±0.06) mg·g−1 were recorded. In contrast, the chlorophyll fluorescence parameters and antioxidant enzyme activities were improved. Conclusion Under 20 h·d−1 of 200 μmol·m−2·s−1 light exposure, the growth of L. leucocephala seedlings in a nutrient solution of 7‰ salinity that contained 15 mmol·L−1 N and 1 mmol·L−1 P rendered the morphological indices, antioxidant enzyme activities, and chlorophyll fluorescence parameters superior to the other tested conditions in a nursery.
2023, 38(2): 193-201.
doi: 10.19303/j.issn.1008-0384.2023.02.009
Abstract:
Objective Effect of Astragalus membranaceus straw on in vitro rumen digestion of cattle was investigated to determine the applicability of Astragalus membranaceus straw in ruminant feed. Methods An experiment was divided into two groups of corn stalk meal (CON group) and Astragalus membranaceus straw meal (AM group) with three repeats per group. Gas production in the in vitro fermentation at 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, 16, 20, 24, 28, 32, 36, 40, 44, 48, 56, 64, and 72 h were monitored. At 72 h, the terminal pH and contents of ammonia nitrogen, volatile fatty acids, and dry matters digestibility were measured. Results (1) In AM group, the amounts of gas production at all sampling points during the fermentation were significantly reduced but the gas production rate was increased (P＜0.01). (2) In AM group, the acetic acid content and the acetic acid to propionic acid ratio were significantly higher (P＜0.01), but the contents of ammonia nitrogen, total volatile fatty acid, propionic acid and butyric acid were lowered significantly (P＜0.05). (3) No significant differences on pH and dry matter digestibility were observed between CON and AM (P＞0.05). Conclusion Astragalus membranaceus straw decreased the total rumen gas production, and had a certain regulatory effect on rumen acetic acid fermentation, which indicated Astragalus membranaceus straw had the potential to be used as feed and feed additive for ruminants.
Effects of Crop/Animal Co-cultivations on Physiochemical Properties and Fertility of Rice Paddy Soil
2023, 38(2): 202-209.
doi: 10.19303/j.issn.1008-0384.2023.02.010
Abstract:
Objective Effects of ecologically friendly co-cultivation of crops and animals on the physiochemical properties and fertility of soil on rice paddy fields were analyzed. Methods Principal component analysis (PCA) was used to study the effects on the soil of paddy fields in South China under 4 types of co-cultivation that combined rice-growing and duck-raising (RD), rice-growing and fish-aquaculture (RF), or rice-growing and shrimp-aquaculture (RS) in comparison to the conventional mono-crop practice on a rice paddy field (CK). Bulk density, porosity, acidification, and nutrient contents of the soil were determined. Result All co-cultivations significantly reduced bulk density, improved porosity, alleviated acidification, and increased contents of nutrients and organic carbon in the soil. Of the 3 methods, RD rendered the greatest porosity, organic carbon, total nitrogen, total phosphorus, total potassium, alkali-hydrolyzable nitrogen, available phosphorus, and available potassium in the 0–10 cm surface paddy soil. The fertility scores on the co-cultivations as ranked by PCA were RD＞RF＞RS＞CK for the surface soil and RD＞RF＞CK＞RS for the 10–20 cm sub-surface soil. The primary factors contributing to the soil fertility included bulk density, organic carbon, porosity, humin carbon, total nitrogen, and alkali-hydrolyzable nitrogen, while the secondary factors, total potassium, pH, available potassium, total phosphorus, fulvic acid carbon, humic acid carbon, and available phosphorus. Conclusion On a paddy field, cultivating rice with ducks (RD), or fish (RF) as the second choice, effectively improved the soil conditions and fertility. Nonetheless, under specific local considerations such maneuver could also be combined with other means to achieve an ecologically beneficial rice farming in South China.
2023, 38(2): 210-219.
doi: 10.19303/j.issn.1008-0384.2023.02.011
Abstract:
Objective A model based on the leaf chlorophyll was established to provide a rapid, convenient, and non-invasive means for evaluating and predicting the quality of varieties of JUNCAOs. Methods Using a multispectral camera, spectra of leaf chlorophyll were obtained from different JUNCAOs. NDSI and RSI vegetation indices were established according to the RGB and HSV color space systems. A chlorophyll meter was used to obtain SPAD readings on JUNCAO leaves . Four regression algorithms were applied to build the model to correlate the spectral and SPAD measurements. Results A significant difference on SPAD was observed between healthy and dry or dead leaves, but not between different JUNCAO. A significant correlation was found between the leaf SPAD and spectral reflection, especially, the red band of RGB color showing a coefficient of −0.79. The high sensitivity of the red band in distinguishing healthy from withering leaves allowed an accurate and stable measurements by the color systems that ranked as: 5-channel RGB＞HSV＞3-channel RGB. Among the 4 inversion methods, the rank was: random forest machine learning＞support vector machine learning＞stepwise linear regression＞univariate linear statistical. And the best fitting function in predicting SPAD was the RGB-NDSI-RF type, which had a R2 of 0.95 vs. 3.04 for RMSE and 0.19 for MRE and validation R2 of 0.75 vs. 8.26 for RMSE and 0.76 for MRE. Conclusion As JUNCAO is becoming increasingly popular for wide applications, a reliable, rapid, non-invasive method to determine the plant quality was much desired. The current leaf chlorophyll spectra-based RGB-NDSI-RF model built by the random forest machine learning method demonstrated an applicability for evaluating varieties of JUNCAO.
2023, 38(2): 220-228.
doi: 10.19303/j.issn.1008-0384.2023.02.012
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Objective To study the diversity and structural differences of rhizosphere bacteria and endophytic bacterial communities in Phyllostachys edulis forests during on and off years. Methods Samples of rhizomes and rhizomes roots as well as rhizosphere soils of degree I, degree II and degree IV P. edulis and non-rhizosphere soils were collected in the on and off years. Genomic DNA was extracted from samples, and Illumina high-throughput sequencing technology was used to analyze the diversity of rhizosphere bacteria and endophytic bacterial communities in P. edulis. Results A total of 31 phyla, 49 classes, 108 orders, 212 families, and 472 genera were identified. The dominant phyla in the on-year P. edulis rhizomes and rhizomes roots were α-Amastigotes, and the dominant order Rhizobia. In the off-year specimens, the dominant phylum was γ-Amastigotes, and the dominant order Bacillariophyceae. At phylum level, the abundance of Actinobacteria was higher in the on-year than in off-year rhizomes; and those of Acidobacteria and Methylobacteria were greater in the on-year than in the off-year rhizomes roots; and those of Firmicutes and Bacteroidetes were less in the on-year than in the off-year rhizomes roots. At class and order levels, the dominants included Frankiaceae and Rhizobia of α-Amastigotes in the on-year rhizomes and rhizomes roots as compared with the off-year samples. At the family level, the abundance of Flavobacteriaceae in the rhizomes root of the on-year was greater than that in the off-year samples. At the genus level, the abundance of Bradyrhizobium in the rhizomes root of on-year was greater than that in off-year. However, the abundance of Burkholderiaceae was lower of on-year than off-year. The rhizosphere soil at the forest in either on or off years did not differ significantly on bacterial diversity, but it was higher on the diversity and richness than the non-rhizosphere soil. Conclusion The rhizosphere bacterial community at a P. edulis forest appeared to be more diverse than the non-rhizosphere, although the diversity was not significantly altered between the years of on and off on the P. edulis growth. The dominant bacteria in the rhizomes and rhizomes roots of the plants differed significantly during the on and off years.
2023, 38(2): 229-237.
doi: 10.19303/j.issn.1008-0384.2023.02.013
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Objective Effect of applying Chamaecrista rotundifolia plant materials to decompose as green manure on the microbial communities in red orchard soil was studied. Method In a simulated experiment, red soil collected from an orchard was used as blank control (CK) or as treatments with added P. americanum×P. purpureum (P) or C. rotundifolia (J). After 10-180d of incubation at 25 ℃ and constant humidity, the soils were sampled 9 times for Illumina high-throughput sequencing. Results Significant alterations were observed in the microbial populations at phylum, class, order, family, and genus levels from 10d to 60d. The relative abundance decreased in 80-140d and became similar for all samples by 180d with Proteobacteria, Acidobacteria, and Actinomycetes being the dominant microbes. Proteobacteria populations in P and J became lower than in CK, while Acidobacteria rose at first and then declined as the incubation prolonged. Actinobacteria in J increased during incubation, whereas Proteobacteria, Actinomyces, Chloroflexi, Firmicutes, Bacteroides, and Verrucobacterium in P and J followed a pattern of either 3 or 2 regression function. Both P and J raised the ACE, Chao1, and Shannon indices, but lowered the Simpson index. Conclusion Composting round-leaf, short-lived perennial cassia C. rotundifolia changed the composition and structure and increased the abundance and diversity of the bacterial community in the red orchard soil. However, no significantly different effects induced by the applications of C. rotundifolia and P. americanum×P. purpureum were found.
2023, 38(2): 238-244.
doi: 10.19303/j.issn.1008-0384.2023.02.014
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Objective In this paper, the characteristics of spore production and enzyme production of Aspergillus oryzae 3.042 from solid-state koji-making Hongqu rice wine grains were studied in order to provide theoretical support for the preparation of seasoning liquid from Aspergillus oryzae solid-state fermentation of Hongqu rice wine grains. Method Hongqu rice wine grains was used as the main raw material. Aspergillus oryzae strain 3.042 was used as the starter. The enzyme activities of acidic, neutral and alkaline proteases, protein enzymatic hydrolysis rate, and ammonia nitrogen conversion rate were used as indicators. The researches on Hongqu rice wine grains and bran were studied to make the effects of culture material ratio, fermentation time and other factors on the growth and enzyme production of Aspergillus oryzae clear. Results The suitable ratio of koji culture material is Hongqu rice wine grains∶bran∶water = 1∶0.8∶1.4. Cultured under this condition for 72 h, the amount of spores, acid protease, alkaline protease, neutral protease of koji , glutaminase were up to 9.93, 1160.94 U·g−1, 1157.47 U·g−1, 1788.40 U·g−1, 10.49 U·g−1. The amino acid nitrogen, enzymatic hydrolysis rate and ammonia nitrogen conversion rate in the crude oil obtained by the koji fermentation were 8.2 g·L−1, 63.40% and 37.08% respectively.Used Hongqu rice wine grains as culture material ratio. The crude sauce has a high flavor, red brown color and luster. The sauce is rich in flavor and has a typical soy sauce style. Conclusion Hongqu rice wine grains are suitable for solid-state koji-making by Aspergillus oryzae, and the suitable ratio is bran:Hongqu rice wine grains:water =1:0.8:1.4.
2023, 38(2): 245-252.
doi: 10.19303/j.issn.1008-0384.2023.02.015
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Cold storage is the most effective method to reduce postharvest decay and maintain quality of loquat fruit. However, loquat fruit is susceptible to chilling injury during cold storage, accompanied with chilling injury symptoms such as fruit browning and pitting, resulting in quality deterioration and economic loss. Therefore, the study on the mechanism of chilling damage and prevention in loquat fruit has important guiding significance for preservation, storage and cold chain transportation of loquat fruit. This article identifies the affecting factors , major symptoms, physiological metabolic changes, and molecular regulatory mechanisms associated with the injury in loquat fruit, including the effects of cold damage on cell membrane system, reactive oxygen metabolism, soluble sugar metabolism, respiration and ethylene metabolism, etc. It also shows the molecular regulatory mechanism of post-harvest cold damage in loquat fruit, and summarises the technical measures to prevent cold damage in loquat fruit, including low-temperature pre-storage, heat treatment, chemical substances and biological materials. The aim is to provide theoretical guidance for the post-harvest preservation and storage of loquat fruit, and to propose directions for further research on cold damage of loquat in the future.
Cold storage is the most effective method to reduce postharvest decay and maintain quality of loquat fruit. However, loquat fruit is susceptible to chilling injury during cold storage, accompanied with chilling injury symptoms such as fruit browning and pitting, resulting in quality deterioration and economic loss. Therefore, the study on the mechanism of chilling damage and prevention in loquat fruit has important guiding significance for preservation, storage and cold chain transportation of loquat fruit. This article identifies the affecting factors , major symptoms, physiological metabolic changes, and molecular regulatory mechanisms associated with the injury in loquat fruit, including the effects of cold damage on cell membrane system, reactive oxygen metabolism, soluble sugar metabolism, respiration and ethylene metabolism, etc. It also shows the molecular regulatory mechanism of post-harvest cold damage in loquat fruit, and summarises the technical measures to prevent cold damage in loquat fruit, including low-temperature pre-storage, heat treatment, chemical substances and biological materials. The aim is to provide theoretical guidance for the post-harvest preservation and storage of loquat fruit, and to propose directions for further research on cold damage of loquat in the future.
