Optimization and Application of SSR-PCR Reaction System for Macadamia

Five major factors of the SSR-PCR reaction system were optimized for genomic DNA of macadamia (Macadamia spp.) by a single factor design. The volume of optimum reaction system was 20 μL that consisted of 30 mg·L^-1 template DNA, 1.0 U Taq polymerase, 2.5 mmol·L^-1 Mg²⁺, 0.6 μmol·L^-1 primer and 0.3 mmol·L^-1 dNTPs. On 15 germplasms of macadamia using different SSR primers, the system proved to be reliable and stable in the amplification bands with high resolution. Consequently, it seemed adequate for the identification and genetic diversity analysis of macadamia germplasms.