Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain

Samples were collected from dead piglets from a swine farm suffered from severe diarrhea in Fujian,2007.To determine the causes of the disease,a virus was isolated from mestenteric lymphnodes of the piglets with ST cells.Obvious CPEs had shown that this virus could proliferate well in ST cells.TCID50,by Reed-Muench,was 10-6.0·(100 μL)-1.The virus was identified to be TGEV with neutralization test,colloidal-gold strips,electronic microscopy and TGEV N gene(1 149 bp) RT-PCR.In comparison with publicated TGEVs in GenBank,homology of nucleotide and amino acid were 95.4% to 99.8% and 96.1% to 100%,respectively.The result indicated that the isolated strain is TGEV,which was first isolated in Fujian,and named as TGEV-FJ strain.