Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

A.bisporus As2796 is the main cultivar in China.For easily cloning the related target genes,the full-length cDNA library of As2796 was constructed.The library had a content of 3.3×106 cfu·mL-1,an insertion range of about 0.7-4.0 kb,an average cDNA length of 1.5 kb,a recombination efficience of about 82%,and full-length ratio of about 35%.The coding sequences(CDS) of 5 target genes were got from the library by PCR amplification,which proved the validity of the library for amplifying full-length cDNA or CDS of A.bisporus genes in need.