Transgenic Rice with mgfp4 Gene Mediated by Agrobacterium tumefaciens

Plant expression vector,pCAGFPHyg,carrying mgfp4 gene was introduced into rice Minghui 86(Indica Sativa L.) mediated by Agrobacterium tumefaciens.The transgenic rice containing 101 independent clones was obtained.PCR and the southern blot analysis ensured that the exogenous genes were indeed integrated into the rice genome.The result of T1 segregation analysis from T0 rice seeds indicated that most of the transgenic plants were inserted by a single T-DNA.It was confirmed by the fluorescence microscopic and RT-PCR detections that the mgfp4 gene was expressed in the transgenic rice,although no acceptable result of green fluorescence was secured.