Cloning and Construction of Over-expression Vector of OsAPX Gene

A full length cDNA of ascorbate peroxidase(APX) gene was cloned from Oryza sativa L.Japonica Nipponbare with RT-PCR.It was 768 bp including the CDS,and was connected to the plant expression binary vector,pBI121,to construct an over-expression vector of APX gene.The pBI121-APX vector was transformed into indica rice,Duoxi No.1 and Hang No.1,mediating by Agrobacterium tumefaciens EHA105.With this,transgenic plants could be obtained for further study on the function of APX gene in rice storability.