Components of Protein Subunit in Correlation with Plant Organs of Fuhua 5 at Pod-setting Stage
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Graphical Abstract
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Abstract
In order to understand the distribution of protein in plant organs of Fuhua 5 during the pod development,the protein content and component of subunits were analyzed with SDS-PAGE electrophoresis.The results showed that,the number of protein subunits in major organs was differing with the pod development and the number of protein subunits in various organ was following the order of nutleafstem=needlerootshell.The protein components were different among organs with the molecular weight from 32 to 238 Ku;in which,the subunit of 34Ku was displayed in most frequency of various organs.The relative concentrations of total protein subunits in various organs were different.The average relative concentration of nuts was the largest and it of the stem was the smallest.The average relative concentrations of total protein subunits in various organ of Fuhua5 was following the order of nut﹥needle﹥leaf﹥root﹥shell﹥stem.The average relative concentrations of protein subunits were differing between the organs and with different molecular weight.The distribution of relative concentration of protein subunits for root,stem,leaf,fruit,nut and needle was in 0.01-1.84,0.01-1.47,0.02-1.57,0.01-1.94,0.02-1.43 and 0.01-4.59,respectively.In all of protein of two peanut genotypes of root,leaf and nut,except nut80Ku and nut33Ku of Fuhua5,the other subunits were significantly related.Correlation between types of subunits had positive and negative correlation,only positive correlation,only negative significant correlation and single correlation.
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