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LI Yong-ping, XUE Zhu-zheng, LIN Hun, WEN Qing-fang. Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 468-471. DOI: 10.19303/j.issn.1008-0384.2013.05.012
Citation: LI Yong-ping, XUE Zhu-zheng, LIN Hun, WEN Qing-fang. Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 468-471. DOI: 10.19303/j.issn.1008-0384.2013.05.012
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Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.

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    • Abstract
  • Through optimization of the concentrations of Taq DNA polymerase,dNTP,primers and DNA template,SRAP-PCR of Abelmoschus esculentus Linn.was established.The results indicated that,in a 20μL reaction mixture,the optimal concentration of genomic DNA template was 75ng;that of the primers,0.4mmol·L-1;that of dNTP,0.75mmol·L-1;that of Taq DNA,polymerase,1.5U;and that of MgCl2,2.0mmol·L-1.
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