Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae

Polysaccharide is one of the active ingredients extracted fromradix pseudostellariae.In this study,the solid-liquid ratio,temperature,extracting duration and times in crude polysaccharide extraction of radix pseudostellariae were investigated by hot water bath,using both the single-factor test and L9(34)orthogonal test.The results showed that the optimum parameters for extraction were with the ratio of solid to liquid 1∶20,extracting temperature 100℃,reflux extraction time 90min and extraction for 3times.The deproteinization effects were compared among three methods,the Sevag reagent,trichloroacetic acid,and trichloroacetic acid-butyl alcohol,and the results presented that trichloroacetic acid-butyl alcohol was the best for deproteinization,with 81.2% crued polysacchsrides and 9.93% protein,indicating that the deproteinization rate was 60.14%.