cDNA Cloning and Sequencing for Precursor of Fujian Yellow Rabbit Follicle Stimulating Hormone β Subunit

The total RNA of the Fujian yellow rabbit′s pituitary was used to amplify the cDNA for the precursor of its follicle stimulating hormoneβsubunit (FSHβ) by means of RT-PCR.The opening reading frame of 390bp was obtained.The fragments amplified were ligated with pMD18-T vector and transformed into DH5αcompetent cells.Randomly selected positive clones were sequenced and submitted to GenBank for verification (accession number: KC784943) .It was compared with homologous sequences of the mammalian animals, including human, cow, sheep, buffalo, and mouse.The sequence alignment showed the similarities ranging from 72.2%to 96.5%.The genetic evolution analysis on the gene indicated a fairly high degree of homology of the rabbit with the primates.The cDNA sequences of the Fujian yellow rabbit′s FSHβwere also compared with those of the Japanese white rabbit′s.Nucleotide mutations at C81Tand G219Awere found, but not on amino acids.