Molecular Identification and Rapid Detection of Pathogenic Plesiomonas shigelloides from Ctenopharyngodon idellus

A Gram negative pathogenic bacterium named strain LCCiL90625NA was isolated from the liver samples of diseased Ctenopharyngodon idellus and was indentified by phylogenetic analysis of 16SrRNA.A phylogenetic tree was constructed based on 16SrDNA sequences of strain LcCiL90625NA and other related bacteria species in the GenBank.It revealed that strain LcCiL90625NA shared a high similarity of 99.7% with Plesiomonasshigelloides and formed a cluster with strain PIC3 (accession number GQ359957) .The isolated bacterium strain LcCiL90625NA was recognized as Plesiomus shigelloides.PCR primers were designed to amplity a 280-bp flagment from the Plesiomusshigelloides23SrRNA gene.The results indicated that this method might serve as an efficient tool for rapid and sensitive identification of Plesiomus shigelloides.