Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector

Transduction of rice blast resistance gene Pi-9 for rice was studied with Sanming dominant genic male sterile rice as the vector and the rice blast resistance 75-1-127 with Pi-9 gene as the donor.Pi-9 gene was introduced into the rice restore Shuangkangmingzhan by successive backcrosses with supporting molecular markers.The results indicated that the pure lines of HP304 with Pi-9 gene similar to Shuangkangmingzhan had been selected from the fertile plants of BC4F3.The basic genetic background of HP304 had been proved to be the same to that of Shuangkangmingzhan. The main agronomic traits of HP304 had no significant difference with Shuangkangmingzhan.And the resistance identification in field showed that the resistant level of HP304 was significantly higher than that of Shuangkangmingzhan.The above demonstrated that it was available to transfer Pi-9 gene into rice using Sanming dominant geneic male sterile as the vector.