A Simple and Rapid Applicable Protocol for Extracting High-quality Total Proteins from Rice

A simple and rapid applicable protocol for extracting high-quality proteins from rice tissues is described.In the protocol, precooling methanol and acetone is used to precipitate proteins and remove contaminants and secondary metabolites.Adding protease inhibitors in methanol for washing is include all proteins to maximize and reduce degradation of proteases.By using this protocol the total protein yield of different tissues were range from 20 to 99mg·g-1FW and the entire process taken 1.6h.To evaluate the quality of the extracted protein, SDS/PAGE gel and Western Blot were employed.4 antibodys represented different protein type include the nucleoprotein antibody, plasmosin antibody, membrane protein antibody and granule-bound starch protein antibody as first antibody for Western Blot.The results show the extraction of high-quality protein samples suitable for Western analysis, and the overall quality of the protein profiles was good.The simplified protocol described here presents considerable savings of time and reagents.