Orthogonal Optimization for Propagation of Clustered OncidiumBuds

Peduncle axillary buds of the Little Cherry, Oncidium, were induced as explants.The orthogonal design was used to evaluate the effects of the culture medium containing inorganic substances, 6-BA, NAA and LH on the propagation of Oncidiumbuds.The result showed that 6-BA, the base medium, NAA and LH affected significantly on the bud multiplication.The optimal medium was the Improved Formulation No.1, which consisted of the base medium, 6-BA 3.0mg·L-1, NAA 0.1mg·L-1 and LH0.5g·L-1, and the average propagation coefficient was 6.53 in 45days.The breeding efficiency was effectively improved.The result appeared to provide a basis for the commercialization of the seedling propagation.