Phylogenetic Diversity of Russula griseocarnosa from Fujian

Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity.Two gene regions of ITS (internal transcribed spacer) and RPB2 (the second largest subunit of the nuclear RNA polymerase enzyme II) were cloned.The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species.It showed that, based on the ITS sequences, R.griseocarnosafound in the province clustered with R.griseocarnosa X.H.Wang, Zhu L.Yang & Knudsen, sp.nov.found in Yunnan, with a bootstrap value of 99%.The differences among R.griseocarnosa, R.vinosa and R.rosea were apparent, with a bootstrap value below 50%.The maximum parsimony tree based on the RPB2 sequences also showed that R.griseocarnosa clustered with R.griseocarnosa X.H.Wang, Zhu L.Yang & Knudsen, with a bootstrap value of 94%.The distinction was also apparent, with a bootstrap value less than 50%.