Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton

Plantlets of Fuzhou jasmine, Jasminum sambac (Linn.) Aiton, were obtained by using the stems and floral organ of the plant as explants.The callus induction, subculture and long-term maintenance of the plantlets were investigated on media with varied concentrations of phytohormone.The results indicated that the optimal light density for the culture was 1 500-2 000lx, and that 6different types of callus could be formed by varying the phytohormone concentration.The medium consisting of MS, 2.0mg BA/L, 0.2mg NAA·L-1 and 30g sucrose ·L-1 yielded a 100%induction rate, and at the same time, was the best for the callus subculture multiplication, as well as the growth of the induced plants.