ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus

51samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) .The results showed that 24samples were SPFMV-positive, the positive rate were 47.06%;the highest positive rate was 71.40%, detected in the samples from Quanzhou, and the next were 50.00%, detected in samples from Nanan and Longyan.A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV, and a specific RT-PCR detection procedure was established.The amplified SPFMV-specific fragment in PCR contained 441bps, and showed 92%-97%, homology with the SPFMV reference sequence.