Research on Tissue Culture and Fast Propagation Technology of Sapindus mukurossi

Vigorous young stem segment of Sapindus mukurossi were used as explants for tissue culture and fast propagation.The results showed that the optimum medium formula of bud induction culture was MS + NAA 0.2 mg·L-1+IBA 0.05mg·L-1+6-BA 3.0mg·L-1and the bud rate reached 94.01%.The optimum combination of multiplication culture was MS+IBA 0.02mg·L-1+6-BA 1.0mg·L-1.During rooting culture,1/2MS+ IBA 0.5mg·L-1+ NAA 0.1mg·L-1 was the optimum medium.The highest rooting rate is 80.47%.After refine seeding transplant,the survival rate was maximal for 89.21%.