RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice

Based on the previously obtained overexpression transgenic plants with the ubiquitin ligase, OsHUB2, from rice, expression lever of the enzyme was studied.Three pairs of primers, OsHUB2-F1R1, OsHUB2-F2R2 and OsHUB2-F3R3, were designed using the Primer Premier 5.0according to the sequence of the enzyme from GeneBank.Subsequently, RT-PCR was conducted to select the best primer pairs with a specific and single amplification product.The results showed OsHUB2-F3R3 to be desirable on both and functions.The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase.