Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae

The SrDFRgene cDNA sequence involved in flavonoids synthesis was cloned from the blue petals of strelitzia reginae using RT-PCR techniques.The cDNA sequence consists of 246 bp, encoding apolypeptide of 82 amino acids.Homology analysis showed that the deduced SrDFRprotein was highly homologous to other DFR proteins from different plants.Phylogenetic analysis indicated that SrDFR was clustered together with DFR of Curcuma alismatifoliafirstly and had close genetic relationship.The semi-quantitative PCR analysis indicated that SrDFRshowed the highest transcript abundance in e and SrDFR was highly expressed in blue petals, but lowly expressed in leaves and yellow sepals.The results indicated that SrDFRplayed an important role in the growth of blue petals.