Adaptation of Goose Parvovirus to Muscovy Duck Embryo Fibroblast

In order to study the adaptation and propagation of goose parvovirus (GPV) in Muscovy duck embryo fibroblast (MDEF) , cultures through serial passages, and PCR assay, indirect immunofluorescence, flow cytometry were used to study the biological characteristics of GPV.Specified genomic nonstructural protein (NS) gene of GPV with the segment lengths of 734 bp was amplified by PCR, and PCR assays showed that the cell cultured 65 h after inoculate with 10-6 dilution of virus suspensions could be recognized specifically.Immunofluorescent studies showed that the infected MDEF had fluorescence characteristics using purified rabbit polyclonal antibody against GPV.The flow cytometry assay demonstrated that the GPV propagated slowly in MDEF, and GPV infection would induce the apoptosis in early stage.The results indicated the GPV strain adapted to and propagated in MDEF without cytopathic effects (CPE) on the cultures.The study provides a basis for cell dependent correlation research of GPV.