Prokaryotic Expression of E Protein from Duck Tembusu Virus

The entire E gene from the duck Tembusu virus (strain WR) was amplified by RT-PCR, and cloned into the pEASY-T3 vector.The recombinant plasmids carrying the target fragment were digested with Bam HI and Xho I, and cloned into the pET-32 avector to construct recombinant plasmid to be named pET-E.pET-E was subsequently transformed into Escherichia coli BL21 (DE3) .The fusion protein was expressed by IPTG induction, and presented mainly as inclusion bodies.The positive western blotting result suggested a strong reactinogenicity of the protein.