Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato

A duplex PCR protocol for the detection of two main viruses in sweet potato was developed based on the coat protein (CP) gene of Sweet potato feathery mottle virus (SPFMV) and the (Hsp70) gene of Sweet potato chlorotic stunt virus (SPCSV) .They were able to amplify unique DNA fragments of 461 bp and 304 bp in a single PCR reaction.The sequence analysis suggst that the two viruses shared at least 93 percent homology with other related viruses.