The Influence of the Fatty Acid Extraction Method on the Fatty Acid Determination of the Bacillus Species

Fatty acids were important components of cell membrane of bacteria,and were subjected to the control of genetic material on cell membrane.The components and contents of fatty acids of Bacillus species were highly conserved and stable.Differences in chain length,position of double bond and binding of functional group,made fatty acids very useful as taxonomic markers.In bacterial culture and fatty acid extraction stages,the experiment results showed that the identification of Bacillus′s fatty acids was affected by saponification time and fatty acid extraction reagent dose.Using type species as object,effects of different detection conditions on fatty acid results of the genus Bacillus were investigated.The optimum conditions for the detection were saponification time of 25 to 30min,saponification reagent dosage of 1.0mL,methylation reagent dosage of 1.5mL.