Establishment of Tissue Culture System for Siraitia grosvenorii

The stem segment of Siraitia grosvenorii was used as the material for establishing of tissue culture system with micropropogation.The results showed that the medium of MS + 6-BA 1.0mg·L-1+ NAA 0.10mg·L-1+sucrose 30g·L-1+agar 6.0g·L-1 was suitable for inducing axillary bud with induction rate of 40.0%.Single nodes cutting and accelerating for axillary buds growth were applied in subculture and optimal medium was 2/3in modified MS + IBA 0.5 mg·L-1+ GA3 0.3 mg·L-1+ sucrose 40g·L-1+ agar 6.0 g·L-1,with proliferation coefficient 4.0.The optimal rooting medium was 1/2in modified MS +IBA 0.5mg·L-1+ sucrose2.0% + AC 0.1% + agar 6.0g·L-1,with rooting rate of 94%.The suitable transplanting medium was peat soil+ pearlite in the ratio of 10∶2,with survival rate for 97.8%.