Isolation and Identification of Mycoplasma ovipneumoniae FJ01-CL

To confirm a suspected contagious pleuropneumonia case at a sheep farm in Fujian,the pathogen from the diseased sheep lung tissue was isolated and purified on a mycoplasma medium.It was subsequently identified by using a biochemical test and specificity polymerase chain reaction(PCR).There were " mulberries,no center umbilical" colonies on the petri dishes observed in the laboratory.The isolated FJ01-CL could ferment glucose,but could not hydrolyze arginine,nor decompose urea.It was positive on the Meilan reduction reaction,the 4-azolenitrogen-chloride reduction reaction,the blood adsorption test and the cholesterol test.The PCR amplification of the sheep mycoplasma pneumoniae showed a specific band of 361 bp.Compared with 8sequence strains from the Genbank,the sequence analysis indicated that the isolated FJ01-CL exhibited 98.1% homology with the reference strain,Y-98,and 98.7% with the local strains,MoGH3-3.The results suggested that Mycoplasma ovipneumoniae FJ01-CL was the pathogen that caused the caprine mycoplasmal pneumonia in the province for the first time.