Cloning and Expression Analysis of Flavonoid 3-O-glucosyltransferase Gene from Narcissus tazetta.var.Chinensis

The Flavonoid 3-O-glucosyltransferase gene was cloned from Narcissus tazetta.var.Chinensis by RACE and RT-PCR.The full lenght of Nt3 GT was 1 682 bp with an ORF of 1461 bp,which encoded a protein of487 amino acids with a conservative domain of Glycos_tranf₁superfamily.Phylogenetic analysis demonstrated that Nt3 GT was in the closest relationship with Solanum tuberosum(AAZ76827.1),Solanum tuberosum(AAX63403.1)and Solanum pinnatisectum(AAY88192.1).Semi-quantitative RT-PCR revealed that Nt3 GT could expressed in leaves and flower,and the expression in leaves was stable in different times.However,the expression in buds was unstable.It could not be detected during scape-sprouting,and was the highest in flowering.