Saccharification of Erianthus arundinaceum Fibers by Liquid Enzymatic Digestion

Liquid digestion of Erianthus arundinaceum fibers in the solution containing 2%NaOH and 1%H2O2to achieve the desired cellulase saccharification was studied.Effect of the substrates,digestion time,pH,fiber content,temperature and agitation speed on the process were analyzed.The conditions studied for the fiber hydrolysis included the applications of 2% NaOH and 1%H2O2,28 to 30hof reaction time,pH4.5to 5.5,4to 6gof fiber,temperatures between 50℃ and 55℃,and agitation speeds of approximately 180r· min-1.The orthogonal experiment indicated the optimal conditions to reach a saccharification rate of 45.21% were 30 h,pH6.0,5g fiber and 52℃.