Clinostat and UV Treatments for Bergenin Synthesis in Callus of Ardisia crenata Sims.

A lab-designed clinostat and a UV lamp were applied to enhance the synthesis and accumulation of bergenin in the culture of Ardisia crenata Sims.calli.The simulated microgravity significantly increased the bergenin content with a peak amount 1.85 times of that of control.It also hastened the bergenin formation shortening the time to reach its peak in the calli.The exposure of calli to UV at 308 nm during the early stage of the culture benefited the bergenin accumulation.Both treatments appeared to induce varied effects in promoting the bergenin synthesis and accumulation.However,at the same time,they also retarded the callus growth.