Advance in Methodology Development for Detecting Vibrio parahaemolyticus

Vibrio parahaemolyticus(Vp)is a halophilic gram-negative bacterium.It widely exists in warm water fishes,shrimps and shellfishes.The seafood carriers,when ingested,can cause the typical gastroenteritis symptoms including diarrhea,intestinal cramps,nausea and vomiting.When a patient is seriously infected,septicemia and kidney failure may result.The rate of the food poisoning incidents caused by Vp has increased drastically in recent years,reaching the top of the list among all foodborne illnesses.With the wide-spread nature and seriousness of the diseases in humans,the need for a rapid,sensitive,low-cost and effective detection method has become apparent.Methodologies based on the virulence factors that apply the molecular biology techniques including PCR,Loopmediated isothermal amplification,nucleic acid probe and gene chip,as well as the immunology tools,such as ELISA and the immune colloidal gold,are being explored.This article evaluates the relevant methods to provide a comprehensive reference for the related studies.