Isolation of Pathogen and Fungicide Selection for Sclerotial Blight in Dendrobium officinale

The pathogen that causedSclerotialblight in Dendrobium officinaleKimura et Migo was identified to beSclerotiumrolfsii Sacc.by its morphological characteristics,as well as through the pathogenicity test and sequence homology analysis of its rDNA-ITS.The ITS sequence of the funguswas 100% identical with that of Atheliarolfsii(GenBank ID:HQ420816.1).Subsequently,a toxicity test on 5fungicides for controlling S.rolfsii was conducted in the laboratory by using mycelium growth rate method and variance analysis.The results showed thatpolyversum and 80% ethylicin hada significant effect on controlling the pathogen with aninhibitory rate exceeding 87%,which reached an extremely significant level.