Screening and Phylogenetic Analysis on A Protease-producing Bacillus Species

LIU Guo-hong; LIU Bo; CHEN Zheng; LAN Jiang-lin; CHE Jian-mei; CHEN Qian-qian; LI An-qi

doi:10.19303/j.issn.1008-0384.2015.10.007

LIU Guo-hong, LIU Bo, CHEN Zheng, LAN Jiang-lin, CHE Jian-mei, CHEN Qian-qian, LI An-qi. Screening and Phylogenetic Analysis on A Protease-producing Bacillus Species[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 954-957. DOI: 10.19303/j.issn.1008-0384.2015.10.007

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Screening and Phylogenetic Analysis on A Protease-producing Bacillus Species

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Bacteria isolated from the soil around a lake in Xizang(Tibet),China were studied.Physiological and phylogenetic analysis were conducted to understand the diversity and ecological distribution of the psycrotrophic and psycrophlic populations in the area.On NA media,the collected cold-adapted strains of bacteria that produced protease were cultured.Their taxonomic identity and genetic variability were determined according to their partial16 SrRNA gene sequences.Of the 16 isolates,FJAT-24893 was found to exhibit a high protease activity.It was subsequently identified as Bacillus frigoritolerans.The optimal protease-producing conditions for culturing the strain were determined to be 25℃and pH8.

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Screening and Phylogenetic Analysis on A Protease-producing Bacillus Species

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