Diversity of Endophytic Fungi in Leaves of Dendrobium candidum

To determine the diversity of the endophytic fungi grown on Dendrobium candidum,the ITS2 region from the samples was sequenced using the Illumina MiSeq high-through put sequencing method.The number of sequences and operational taxonomic units(OTUs)of each sample were sorted and calculated using Qiime and Mothur software.The species abundance and distribution,alpha diversity index,and time of species abundance of the fungi were determined.The number of effective sequences was found to be 41 361;and the OTUs was 33.The rarefaction functions indicated an adequate sampling,while the OTU number was close to saturation.It was concluded that the endophytic fungi found in D.candidumbelonged to Emericella,and E.nidulans was the dominant species.