Optimized Preparation for Chondroitin Sulfate from Squid Cartilage by Enzymatic Hydrolysis

Factors affecting the enzymatic hydrolysis with papain in the preparation of chondroitin sulfate from squid cartilage was studied using single factor tests.Subsequently,optimization for the process was carried out with an orthogonal combination design experiment.It was concluded that adding 2% addition of papain to the substrats pH6.5and 60℃for 5hcould yield 4.97% protein and 70.11% chondroitin sulfatefrom the squid cartilage.