Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis

The young,tender flower stalks of the Big Chili Phalaenopsis were used for this study on the tissue culture and rapid propagation of the orchid.Using an orthogonal design experiment,the effects of the basic culture medium as well as hormone application and concentration on the pedicelinduction,proliferation and differentiation of the adventitious buds and the seedling development and rooting of the plant were investigated.The results showed that(a)the best medium for inducing the adventitious buds was Hyponex No.1+NAA(0.3mg·L^-1)+6-BA(5mg·L^-1);(b)the best medium for the proliferation and differentiation of the adventitious buds was Hyponex No.1+NAA(0.2mg·L^-1)+6-BA(6mg·L^-1),which resulted in a multiplication factor up to 5.53times;(c)the best medium forseedling development and rooting was 1/2MS+NAA(0.2mg·L^-1),which yielded a rooting rate of 96%,and coconut milk,apple juice,potato juice as parts of the composite additives were found to be advantageous for rooting;and(d)the survival rate of the transplanted tissue culture was greater than 91.5%.