Restriction Fragment Length Polymorphism of LPL and FASN Genes in Huiyang Bearded Chicken

Restriction fragment length polymorphism (RFLP) of LPL and fatty acid synthetase (FASN) genes in Huiyang bearded chickens was obtained to facilitate the selection for dominant genotypes associated with the high-quality meat and fast-growing characteristics in chicken. Genomic DNA was extracted from the chicken blood. The two genes of interest were amplified by PCR. The amplied product of LPL gene was digested by Hinf I,and FASN gene by Hae Ⅲ, for RFLP analysis. The results showed that (1) in LPL gene,the genotype BB was 76.3%,while the two newly discovered genotypes were 1.7% and 22%, with no AA type detected; (2)in FASN gene, genotype BB, CC, and BC were 64.8%, 23.4%, and 12.24%, respectively,while the frequencies of B and C genes were 0.7041 and 0.2959, respectively.