Prokaryotic Expression of 3 GT Gene from Narcissus tazetta var. Chinensis

Flavonoid 3-O-glucosyltransferase(Nt3GT) gene of Narcissus tazetta var. Chinensis was cloned into the vector, pET29a, to construct prokaryotic expression recombinant plasmid, pET29a-NT3GT.The recombinant plasmid was transferred to E.coli BL21(DE3) to be induced by IPTG. Subsequently, the expression of the target protein was verified by SDS-PAGE. Then, pET29a-Nt3GT was successfully constructed, which induced the 55 kDa recombinant protein of Nt3GT in the prokaryotic expression system. Nt3GT was cloned, and its vector was constructed and induced to be expressed successfully by IPTG in BL21 providing a basis for theantiserum preparation and functional analysis in the future.