Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian

To study the ORF3 gene of porcine epidemic diarrhea virus (PEDV) in Fujian, samples were collected from regions in the province during 2014-2015. Eight strains were amplified for sequence alignment and phylogenetic analysis. Genetic evolution analysis showed that the strains were of wild variety and close to those found domestically and in the neighboring countries in recent years with up to 100% homology on the nucleotide and deduced amino acids. But, they were distantly related to the classical strain of CV777, with a nucleotide homology of 96.0%-96.9% and amino acid homology of 92.5%-94.7%. It appeared that genetic alternations had occurred to the PEDV strain currently isolated from the samples collected in the province.