Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian

Based upon the characterization of gB gene retrieved from GenBank, specific primers were selected to amplify the gB gene of porcine cytomegalovirus (PCMV) FJ01 isolated in Fujian. The target PCR fragments were then cloned and sequenced. The gB gene was found to have 2 580 nucleotides, encoding 859 amino acids. Its homologies on nucleotide and amino acid sequences with the data at GenBank were greater than 98.3% and 97.9%, respectively. In addition, nearly half of the PCMV isolates had a same ACA nucleotide deletion in their gB genes that correlated to the phylogenetic evolution of the isolates, except ZZ strain.