Cross-species Applicability of EST-SSRs from Capsicum annuum L.

The applicability of the EST-SSRs marker identified on Capsicum annuum L for uses on other solanaceae vegetables was investigated. A total of 118, 900 ESTs in C. annuum L were downloaded from NCBI. Two hundred pairs of EST-SSR primers were developed. Approximately 98%, 94.5% and 90.5% of them were successfully amplified in C. annuum L., Solanum lycopersicum and S. melongena L., respectively, with the polymorphism proportions of 35.5%, 29.5% and 26.5%, respectively. The polymorphic primers specific to the parents and their F1 of C. annuum L., S. lycopersicum and S. melongena L. were tested with the anticipated results. It appeared that the SSR primers developed from the EST database related to C. annuum L. could be applied on solanaceae plants with satisfaction.