Rapid Propagation of Dendrobium spp. Using Tissue Culture

Newly geminated buds of Dendrobium spp., Sanya Sunny, were used asexplants to induce bud clumps. Effects of culture media (i.e., Hyponex #1, Improvement #1, Improvement #2, and Improvement #3), phytohormones (i.e., 6-BA, NAA, and IBA), and sugar on the bud clump induction, propagation, and rooting of the plantlets were analyzed and optimized using anorthogonal experiment. The results showed that the major factors affecting the bud multiplication were medium, 6-BA, NAA, and sugar. Improvement #2 medium that contained 1.0 mg·L^-1 of 6-BA, 0.2 mg·L^-1 of NAA, 30.0 g·L^-1 of sugar, 3.0 g·L^-1 of agar powder and 3.6 g·L^-1 of carrageenanprovided the best average propagation coefficient of 6.25 in 50 d among all tested media. For rooting, Improvement #3 with added NAA at 0.3 mg·L^-1, IBA at 0.3 mg·L^-1, AC at 0.5 g·L^-1, banana puree at 100.0 g·L^-1, sugar at 20.0 g·L^-1, agar powder at 3.6 g·L^-1 and carrageenan at 3.6 g·L^-1 performed best with a 100.0% rooting rate. The survival rate of the plantlets in 60 d after transplanting from test tubes was 96.5%.