SSR Loci in Transcriptome of Moringa oleifera Lam

MISA software was employed to analyze the distribution and basic characteristics of SSR loci of 15 824 unigenes, which were longer than 1 kb, from the transcriptome of Moringa oleifera Lam leaves at different development stages. A total of 8 272 SSR loci consisting of 114 motifs were detected in 6 003 unigenes, i.e., a frequency of 52.28%. The most abundant repeating motif was a single nucleotide (55.69%), which was followed by dinucleotide (32.34%) and trinucleotide (11.24%); and, their SSRs were A/T, AG/CT and AAG/CTT, respectively. The most commonly found SSRs had 10-20 tandem repeats (30.35%) with their motif lengths ranging from 12 bp to 19 bp. It appeared that there were numerous SSRs in the transcriptomes with high distribution frequencies and many varieties. The results obtained from this study provided valuable information for developing SSR markers.